Potassium Diformateas Feed Additive to Enhance the Performance of Immune System on Giant Gourami Fingerlings (Osphronemus goramy Lacepede, 1801)

This research aims to determine the optimum dose of potassium diformate (KDF) which is added to commercial feed to improve the immune performances of giant gourami fingerlings. The research was conducted in October – December 2019 in Aquaculture Laboratories and Biotechnology Laboratory of the Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran. The method used in this research is Completely Randomized Design (CRD) with four treatments and four replications. The treatments are addition of potassium diformate in feed with a dose of 0% (A), 0,3% KDF (B), 0,5% KDF (C) and 0,8% KDF (D).The giant gouramimaintained for 40 days. The observed parameters are total leukocytescount, total erythrocytescount, differential leukocytes, and water quality. The data of total leukocytes, total erythrocytes were analyzed using ANOVA and followed by Duncan’s Multiple Range Test, while differential leukocytes and water quality are analyzed descriptively. The results showed that the addition of 0.3% potassium diformate is the most effective to improve the immune performances of giant gourami fingerlings. During 40 days of treatments, total leukocytes count increased 25% from6.55×104 cells/mm3 to 8.74×104 cells/mm3, total erythrocytes countincreased 10.03% from 1.28 ×106 cells/mm3 to 1.43×106 cells/mm3. Potassium diformate treatment 0.3% was an effective dose to stimulate differential leukocytes of gourami fingerlings are triggered to enhance the immune system.