Larvicidal Potential of Conidia Suspension of Aspergillus flavus against Anopheles sp.

Aim:This study was aimed to investigatethe larvicidal potential of the conidia suspension of Aspergillus flavusagainst Anophelesmosquitoes.Methods: Aspergillus flavus was isolated from soil using soil suspension procedures and was identified using morphological characteristics. Bioassay was performed to determine the efficacy of Aspergillus flavus conidial suspension against early 4thinstar larvae of Anophelesmosquito using WHO-2005 protocol with slight modifications.Results:Four different concentrations of conidial suspension; 3.3×106, 3.3×105, 3.3×104and 3.3×103conidia/ml were tested, and the results showed that; mortality increases with the increase in conidial concentrations and exposure time. The lowest mortality (12%) was recorded at 3.3×103 conidia/mL and 24-hours post exposure whereas the highest mortality (78%) was recorded at 3.3×106conidia/mL and 72-hours post exposure. LC 50% and 90% for the larvae was found to be 1.6×108and 4.2×109conidia/mL at 24-hours; 2.0×104and4.0×105conidia/mL at 48-hours; 1.3×103and 3.2×104conidia/mL at 72-hours.Conclusion: These results indicated that Aspergillis flavus conidia suspension are pathogenic to immature stage of Anophelesmosquito and could be suggested as a biological control for mosquito management.Original Research Article