Estimation of Ursolic Acid in Bauhinia racemosa Lam. by High-performance thin Layer Chromatography Method

Plants have been utilized since ancient times to cure and manage a variety of diseases. Their capacity to heal or prevent ailments is emanates from the presence of various phytochemicals such as alkaloids, flavonoids, glycosides, terpenoids, steroids, tannins, phenols, etc. Pentacyclic triterpenoids have attracted the interest of researchers due to their diverse pharmacological activities. One of the Pentacyclic triterpenoids is Ursolic acid (UA) with significant biological effects, including anti-inflammatory, anticancer, antidiabetic, antioxidant, and antibacterial properties. Bauhinia racemosa Lam. is used in folk medicines for the treatment of various disorders. In the present work, a subtle HPTLC method has been developed for determination of Ursolic acid in Bauhinia racemosa Lam. The demonstrated method is simple, rapid, accurate and can be used for routine qualitative testing.