Stability Indicating UV Spectrophotometric Method Development and Validation of Fisetin in Pure and Pharmaceutical Capsule Dosage Form

Objective: The objective of the study was to develop and UV Spectrophotometric method and apply the method to dosage form.

Methods: A simple, precise and sensitive ultraviolet spectrophotometric method was developed for the determination of Fisetin in pure and pharmaceutical capsule dosage form; the spectroscopic method was run through Shimadzu UV-1800 with solvent of Methanol: 0.1%OPA was used in this method-working wavelength was selected at 362nm.

Results: Beer-Lambert’s law revealed a good correlation in the concentration range of 3-15µg/ml. The absorbance was found to be 0.385 with %RSD for interday precision and intraday Precision was 0.53% & 0.51%.

Conclusion: The developed method was successfully applied to the determination of Fisetin in commercially available dosage forms. A statistical comparison of the results showed an insignificant difference between the proposed method and reference method. The proposed methods offered the advantages of simplicity and economy that can be applied without the need for expensive instrumentation and reagents in quality control analysis.