Beyond Separation: The Transformative Impact of Hyphenated Techniques in Micro and Macro Quantification

This article describes the various hyphenated techniques, a brief note on their instrumentation, and the working principles that are used in the current setup of industries. The hyphenated technique is the combination or the coupling of the different analytical techniques. Mainly chromatographic techniques are combined with spectroscopic techniques. Then the separated components of the mixture from chromatographic technique will enter into the spectroscopic technique through an interphase.

Like-wise their remarkable improvement and efficiency over the past decade. Techniques like single quadrupole inductively coupled mass spectrometry (ICP-Q-MS), ICP- Triple quadrupole mass spectrometry (ICP-QQQ), Liquid Chromatography-Two -dimensional Gas chromatography-mass spectrometry (LC-GCXGC-MS/MS), Two-dimensional liquid chromatography (2D-LC), Fourier transform near-infrared spectroscopy (FT-NIR), etc. are considered as recent improvements in this trend. Creating hyphenated methodologies is beneficial to many scientific domains, such as food and drug analysis and biological research. Apart from the enhancement, significant dimensional alterations have been noted in elemental specifications and natural product analysis. Advances in analytical techniques for the separation and detection of substances have empowered the identification of compounds in complex mixtures, in addition to making it conceivable to acquire the metabolic profile of plants, evading the re-isolation of known compounds by the procedure of dereplication.