The Amyloid Assembly of the Bacterial Hfq Is Lipid-Driven and Lipid-Specific

Turbant, Florian and Machiels, Quentin and Waeytens, Jehan and Wien, Frank and Arluison, Véronique (2024) The Amyloid Assembly of the Bacterial Hfq Is Lipid-Driven and Lipid-Specific. International Journal of Molecular Sciences, 25 (3). p. 1434. ISSN 1422-0067

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Abstract

Under specific conditions, some proteins can self-assemble into fibrillar structures called amyloids. Initially, these proteins were associated with neurodegenerative diseases in eucaryotes. Nevertheless, they have now been identified in the three domains of life. In bacteria, they are involved in diverse biological processes and are usually useful for the cell. For this reason, they are classified as “functional amyloids”. In this work, we focus our analysis on a bacterial functional amyloid called Hfq. Hfq is a pleiotropic regulator that mediates several aspects of genetic expression, mainly via the use of small noncoding RNAs. Our previous work showed that Hfq amyloid-fibrils interact with membranes. This interaction influences Hfq amyloid structure formation and stability, but the specifics of the lipid on the dynamics of this process is unknown. Here, we show, using spectroscopic methods, how lipids specifically drive and modulate Hfq amyloid assembly or, conversely, its disassembly. The reported effects are discussed in light of the consequences for bacterial cell life.

Item Type: Article
Subjects: Academic Digital Library > Multidisciplinary
Depositing User: Unnamed user with email info@academicdigitallibrary.org
Date Deposited: 25 Jan 2024 06:41
Last Modified: 25 Jan 2024 06:41
URI: http://publications.article4sub.com/id/eprint/3129

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