In Vitro Regeneration of Centella asiatica L.

Tissue culture technique has been established as a useful ex-situ conservation strategy for endangered species. This paper describes in vitro regeneration of Centella asiatica L. (Urb.) (Apiacae) a threatened species and preliminary estimation of secondary cell constituents in methanolic extract in vitro regenerated plants. Nodal segments were used as explants from in vitro regeneration on MS media supplemented with BAP (2.0mg/l)+NAA (1.0mg/l). In vitro rooting was induced when MS medium was supplemented with IAA (1.0mg/l). Thin layer chromatographic studies were carried out on methanolic extract of in vitro regenerated plant with reference standards of asiaticoside and madecassoside.