ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF JUNIPER BERRY OIL MEDIATED SILVER NANOPARTICLES

Aim: To evaluate the antioxidant and antimicrobial activity of Juniper berry oil mediated silver nanoparticles.

Materials and Methods: Silver nanoparticle was prepared using Juniper berry oil mediated and confirmed by UV-Visible spectrophotometer. The Juniper berry oil mediated silver nanoparticles (AgNPs) were subjected to diphenylpicrylhydrazyl (DPPH) assay to determine the antioxidant activity. The antimicrobial activity of the synthesized juniper oil mediated silver nanoparticle against Streptococcus mutans, Lactobacillus, Staphylococcus aureus and Candida albicans was tested by well diffusion technique by standard procedure.

Result: The UV -Visible spectroscopy showed a peak at 440nm showing the formation of silver nanoparticles. The Juniper berry oil mediated silver nanoparticles showed a dose dependent increase in antioxidant activity up to 30 μL and after that the increase in concentration did not show any further increase in the antioxidant activity. Staphylococcus aureus and Streptococcus mutans showed good zone of inhibition compared to Lactobacillus. Lactobacillus did not show any dose dependent activity.

Conclusion: Juniper berry oil mediated silver nanoparticles showed good antioxidant activity. It showed good activity against Staphylococcus aureus and Streptococcus mutans compared to the standard antibiotic. The nanoparticle also showed good activity against Candida albicans .Hence, the study conclude that Juniper berry oil mediated silver nanoparticles may be utilized for its antioxidant activity and antimicrobial activity.