ANTIINFLAMMATORY ACTIVITY OF ZINC OXIDE NANOPARTICLES SYNTHESISED USING GRAPE SEED EXTRACT: AN in vitro STUDY

Background: Inflammation is the key finding in any periodontal problem. It is important to devise new topical agents to reduce the inflammation. Zinc oxide nanoparticle synthesized from plant extract will be an eco-friendly, simple and reliable method offering therapeutic options which are safe, free of side effects and effective for a wide range of diseases. In our study we have chosen grape seed due to its protection against oxidative damage, and anti-diabetic, anti-cholesterol and antiplatelet properties.

Aim: The aim of the present study was to analyze the anti-inflammatory activity of zinc oxide nanoparticles synthesized using grape seed extract.

Materials and Methods: Collection of grape seeds and preparing its extract followed by the synthesis of zinc nanoparticles using the prepared herbal formulation. The stable zinc nanoparticles were characterized by UV- Vis spectroscopy, which showed shift peak at 660 nm. The anti-inflammatory activity of zinc nanoparticles prepared using plant extract was investigated by albumin denaturation assay. The results of the test were described as the standard deviation (SD) and analyzed using the UV- Vis Spectroscopy.

Results: The zinc oxide nanoparticles synthesized using the grape seed extract showed highest absorbance at a concentration of 50 μl (84.6%) when subjected to inhibition of albumin denaturation assay to check for its anti-inflammatory activity.

Conclusion: The zinc oxide nanoparticles synthesized using the grape seed extract acts as a potent anti-inflammatory agent with least side effects. Further clinical trials need to be done in the future and can be used as a replacement to the commercially available drugs.