Tolerance Tests of Alcaligenes faecalis BW1 Extract

Aims: To highlight whether metabolites of Alcaligenes faecalis BW1 extract can be administered
orally for their possible antimycobacterial effects.
Study Design: Study of the influence of certain parameters on the extract of Alcaligenes faecalis by
using either discs or well diffusion methods against M. smegmatis.
Place and Duration of Study: Laboratory of Microbial Biotechnology, Department of Biology, Faculty
of Sciences and Technical, University Sidi Mohamed Ben Abdellah, BP 2202, Road of Immouzer,
Fez, Morocco From April to August, 2012.
Methodology: The impact of acidic pH of gastric juice, bile, hydrogen peroxide, pancreatic enzymes
and lysozyme on the antimycobacterial activity of Alcaligenes faecalis BW1 extract was evaluated by
agar diffusion method. Detection whether or not antibacterial metabolites having a synergistic effect
with rifampicin against M. smegmatis was also explored.
Results: Antibacterial metabolites of Alcaligenes faecalis BW1 extract resist to the action of gastric
pH, gallbladder bile and hydrogen peroxide. In addition, they are not affected by pancreatic enzymes
and lysozyme. Moreover, they have a synergistic effect with rifampicin against M. smegmatis.
Conclusion: Anti-mycobacterial metabolites of Alcaligenes faecalis BW1 extract are compatible with
rifampicin and could be administered orally as antitubercular agents after their purification,
identification in further work.