A Study to Report Significantly Different Adducts for Petroleum Ether Extract of Tabernaemontana divaricata by Atmospheric Pressure Chemical and Electrospray Ionization Methods

Highly selective and versatile analytical techniques have gained importance for extraction,
identification, and quantification of phytoconstituents from plant extracts. Modern techniques
(qualitative and quantitative) have gain importance due to their unique feature of separation of
components and applicability of the vast existence of multiple spectrometry methods availability. The
last trend of coupling liquid chromatography with mass spectrometry (LC-MS) has become an
important useful tool in the metabolic profiling of plant extracts. In the present study, crude petroleum
ether leaf extract of Tabernaemontana divaricata (PETD) was prepared by ultrasonication. Research
team members tried to identify the probable phytoconstituents and their adducts by isolation and
characterization of hyphenated techniques like Atmospheric Pressure Chemical Ionization (APCI) and
Electron Spray Ionization (ESI). Some of the unique components which were noticed presently were a
fragment of malic acid, 1-heptanol, 5-oxo-11-hydroxyvoaphylline, Bergapten, Falcarindiol, Pinoresinol,
Diadzein, Picrotin, Mehranine, Acacetine, Imidacloprid urea, Xanthinin, Miconioside A, Isotenulin,
Viscidulin, Artoflavanone, Flavocommelin, Gedunol, Mosinone A, Azaleatin, Psoralen, Lahoricine,
Neodiosmin, 5-O-β-D-Mycaminosyltylactone, Voachalotine oxindole, and Cycloartenol. The
compounds were found to be belonging to different classes of alkaloids, flavonoids,
phenylpropanoids, triterpenoids, and essential oils. We attempted to identify each component
however some of the unknown components have been reported in the paper. Hence, with this
elucidation, the team believes that a further study using LC-MS/MS or LC-NMR would reveal
confirmatory information about the PETD extract.