Immobilization of BSA onto Graphene Oxide Synthesized from Hydrogenated Diamond-like Carbon

Over the past few years, biomolecules, and Graphene oxide (GO) material studies have increased. The numerous processes for producing graphene oxide are being improved in an effort to offer safer and more efficient substitutes. Even though the Hummers method is one of the oldest, it continues to be one of the most effective ways to make graphene oxide from graphite powder or flake. In this chapter, GO has been synthesized from Hydrogenated diamond-like carbon (HDLC) which is tunable and an atomically smooth surface that can be deposited on a high-surface-area (100) wafer plate. The HDLC films were heated at a fixed temperature of 9000C for 30 min in a high vacuum of 1 × 10-6 torr and oxygenated at room temperature. This annealed HDLC (A-HDLC) was oxidized and described sequentially. FTIR confirms functional groups and Raman measurements confirm the G and D peak by Oxidation of A-HDLC. The surface morphology of BSA immobilized go, Oxidized Graphene, and A-HDLC, were studied by atomic force microscopy (AFM).