Genotype frequency and use of single nucleotide polymorphisms for detection of informative allele by polymerase chain reaction

Nayyar, Ayesha and Ahmed, Suhaib (2020) Genotype frequency and use of single nucleotide polymorphisms for detection of informative allele by polymerase chain reaction. Pakistan Journal of Medical Sciences, 36 (7). ISSN 1682-024X

[thumbnail of 710] Text
710 - Published Version

Download (3kB)

Abstract

Objective: To determine genotype frequency of biallelic single nucleotide polymorphisms and its use in detection of informative allele in donor/recipient pairs (sibling pairs) having undergone haematopoietic stem cell transplantation with various haematological disorders using a PCR based method.

Methods: This descriptive study was conducted at GRC Lab Rawalpindi from Jan 2018 - Oct 2019. A total of twenty donor/ recipient pairs (sibling pairs) were studied for genotype frequency and informativeness of single nucleotide polymorphisms. Genomic DNA was extracted from the peripheral blood and amplification of single nucleotide polymorphisms was done by PCR based method. The amplified DNA was seen by electrophoresis on 6% polyacrylamide gel.

Results: A sharp band of DNA on the polyacrylamide gel indicated a positive reaction. At least two or more informative SNP markers were found in every sibling pair.

Conclusion: Our results demonstrate that PCR amplification of polyacrylamide gel electrophoresis using single nucleotide polymorphism has allowed the successful screening and detection of informative allele in all the donor/recipient pairs. (Sibling pairs). This PCR based assay using SNPs appears to be a quick, simple, reliable and technically feasible method for a use in a Pakistani setting.

Item Type: Article
Subjects: Academic Digital Library > Medical Science
Depositing User: Unnamed user with email info@academicdigitallibrary.org
Date Deposited: 17 Apr 2023 04:59
Last Modified: 26 Feb 2024 04:24
URI: http://publications.article4sub.com/id/eprint/1276

Actions (login required)

View Item
View Item