Effect of Blood Creatinine on Fatty Acid Metabolites in Human Red Blood Cell Membranes

This chapter aimed to describe the correlate creatininemia and uricemia to HNE and MDA levels of Red Blood Cell purified membranes in comparison to those of normal subjects to characterize the effects of oxidative stress. Creatinine is a waste product that is produced by your muscles. It is typically removed through the kidneys. Healthy kidneys filter creatinine out of your blood, and it leaves your body through urine.

The studied population consisted of 10 patients with endogenous hyper- creatininemia and hyperuricemia, respectively ten pathological and ten normal subjects who were recruited from the Health Center of the University of Calabria, Italy. The measurement of total proteins in RBC ghosts and HNE and MDA are conducted on blood samples of patients.

The increase of MDA and HNE levels represent the elevated activities of oxidative stress in human body. Results revealed that both creatinine and uric acid levels affect the ratio of MDA/protein and HNE/protein content on RBC ghosts, suggesting that they may have antioxidant properties at low blood concentrations and oxidising properties at higher concentrations, protecting against oxidative stress. The interaction between concentrations of creatinine and uric acid, that is powerful scavenger of singlet oxygen, slows down the activity of oxi- dative stress in human erythrocyte membranes.